The strong interest in our ready-to-target CD8 binder-lipids sparked a new direction for us: What if the entire process of building targeted LNPs (tLNPs) could be modularized?
We’re now exploring pre-formed, empty tLNPs—a format that would let researchers mix-and-pack their own mRNA payloads for rapid payload optimization, formulation selection and binder screening.
This “mix & pack” idea isn’t completely new (recent paper here: A Post-Encapsulation Method for the Preparation of mRNA-LNPs via the Nucleic Acid-Bridged Fusion of mRNA-Free LNPs), but we’re pushing it into the targeted delivery space—where we think it can unlock major speedups in discovery.
In the simple mix-and-pack experiment below, we used two different empty LNP formulations to generate tLNPs and evaluate cell-specific eGFP delivery—an early demonstration of how fast iteration could look. This type of experiment can be done at 1-5 µg scale.
